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Add 80 l 10 mM HCl, and lyse the cells by freezing and thawing twice
Before L-carnitine, S-5-HT was markedly low in both CFS (83 (57) 36 nmol/l), and hypothyroidism (112 (84) 63 nmol/l)
This not only trivialises the use of unlicensed medicines but actively discourages people from stopping to ask whether whats being offered is legal or appropriate
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