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Tissue samples from lean and ob/ob mice were homogenized in TRIzol reagent, followed by RNA extraction using standard protocols
This is because issues with regular function of the gastrointestinal tract can prevent the proper release and absorption of the vitamin, which can cause a deficiency if not supplemented properly
Proper storage helps maintain stability
Raun, K., Hansen, B., Johansen, N., Thogersen, H., Madsen, K., Ankersen, M., & Andersen, P
Late phase maturation (weeks 7-12+) Tissue maturation continues beyond acute healing, with collagen cross-linking and fiber organization improving progressively
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