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The autophagy activator rapamycin not only ameliorates fluoride-induced autophagic flux blockage by correcting autophagic degradation impairment but also inhibits fluoride-mediated ferroptosis by reducing the iron content and suppressing lipid peroxidation

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Gentle swirling minimises this interface disruption by keeping the peptide submerged throughout reconstitution, preserving the copper coordination geometry required for monomeric dissolution.