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In this study, we induced senescence in A375 cells using doxorubicin and confirmed cellular senescence through senescence-associated beta-galactosidase (SA--gal) staining (Figure

Research demonstrates that copper-binding peptides including GHK-Cu suppress the NF-B pathway through direct molecular interactions, with nuclear translocation of NF-B p65 decreased by approximately 30% upon treatment and molecular modeling showing specific peptide sequences exhibit high binding affinity to NF-B p65 (up to -8.8 kcal/mol)

The absolute standard curve was constructed using 10-fold serial dilutions of previously purified pIRES2 plasmid

2009 Camarena L, Poggio S, Garcia N, Osorio A: Transcriptional repression of gdhA in Escherichia coli is mediated by the Nac protein

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