-tubulin (rabbit polyclonal 1:1000 Cell Signaling, Cat# 2144, Lot#8) and GAPDH (rabbit polyclonal 1:1000, Cell signaling Technology) and -tubulin (rabbit polyclonal 1:1000 Cell Signaling, Cat# 2144, Lot#8) used to assess equal amounts of protein loaded in each lane
Glutathione (GSH) is an antioxidant and works against the damage to important cellular components caused by reactive oxygen species such as free radicals, peroxides, lipid peroxides, and heavy metals
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Elevated insulin levels cause an increase in insulin-like growth factor (IGF-1) and a decrease in insulin-like growth factor-binding protein 3 (IGFBP-3) (182)