glutathione colorimetric assay Sigma-Aldrich Kit, Fluorimetric, sufficient for 200 multiwell tests 1 kit Diabetes Research Glutathione Peroxidase (GSH-Px) Activity Assay

Marchan : formal analysis, methodology, writing-review and editing

Both MTX and 7-hydroxy MTX are excreted by the kidneys, and a small part is also excreted in the bile (Reference Saka and Aouacheri2)

Caspases assume a pivotal role in apoptosis, serving as primary executors through the cleavage of cellular proteins, thereby instigating and executing the apoptotic cascade

La activacin de esta va aumenta la expresin de enzimas antioxidantes como: Glutatin peroxidasa Superxido dismutasa Catalasa 4) Funciones fisiolgicas del sistema NAC-glutatin Control del estrs oxidativo El glutatin es uno de los principales mecanismos de defensa frente a las especies reactivas de oxgeno (ROS)

References 1 AliS.RasoolM.ChaoudhryH.N PushparajP.JhaP.HafizA.et al (2016)