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In the H&E staining assessment, we applied the INHAND five-tier grading system [24], a commonly used pathological scoring method, to classify inflammatory cell infiltration severity

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Histological examination of skin sections with HE and Masson staining presented insights into the morphology of skin layers and collagen extent during the healing process (Figure 2)

Vitamin B12 deficiency can be due to various reasons such as improper diet or diet deficient in vitamin B12, poor absorption from the intestine, certain disorders of intestine and surgeries which can impact absorption of this vitamin