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For immunofluorescence (IF) staining, after deparaffinization and antigen retrieval, sections were incubated overnight at 4 C with a mouse anti-insulin antibody (1:200, GB15334, Servicebio), rabbit anti-PPAR antibody (1:50, ab23673, Abcam), or rabbit anti-UCP2 (1:200, DF8626, Affinity) antibody, followed by incubation with appropriate secondary antibodies (1:1000, 7074 S and 7076 S, CST)
It also generates foam that makes it impossible to verify whether the powder has fully dissolved
Bariatric surgery may be a good fit for adults with higher BMI and obesity-related conditions who are ready for a procedure and ongoing nutrition support, including daily vitamin and mineral supplements and periodic lab checks to make sure nutrient levels stay healthy
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