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Seek immediate medical attention if you experience severe symptoms, rectal bleeding or pain, do not have a bowel movement within 30 minutes of the coffee enema, or have symptoms of dehydration these could signal more serious potential effects of coffee on the body

Assessment of erythrocyte glutathione peroxidase activity (GPX1 activity) The GPX1 activity of red blood cell hemolysate was assessed with a colorimetric assay using the Bioxytech kit (OxisResearch)