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Cells were centrifuged and resuspended in 0.1 M sodium cacodylate buffer and post-fixed for 1 h with 1% osmium + 0.1 M sodium cacodylate buffer, then dehydrated and embedded in Agar 100 resin

We then envisioned two further scenarios that could explain the differential Nt 7 compartmentalization: (i) PATs have no specificity for substrate recognition, and substrates concentrate at different compartments due to specific interactions, and (ii) PATs discriminate substrates, which in turn accumulate at the compartment where S-palmitoylation takes place

The EORTC 10041/BIG 03-04 MINDACT trial is feasible: results of the pilot phase

Healthy aging requires a balance between energy production, cellular repair, and protection from damage

While waiting, make sure theyre breathing and place them on their side (recovery position) to prevent choking