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You should start with a low dose of DHEA every 2-3 days, increasing to daily as tolerated

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The gradient began at 1% B and ramped to 5% B in 0.1 min, increased to 30% B in 12.1 min, increased to 40% in 0.7 min and finally increased to 99% B in 0.1 min and was held for 2 min to wash the column for a total run time of 15 min