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Output: differential feature list, volcano plot, pathway enrichment map, top-20 annotated metabolites with HMDB IDs

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Skin reactions or injection site effects may occur with B12 supplementation but are not caused by elevated serum levels

Common side effects include: Injection site reactions mild pain, redness, swelling, or bruising at the injection site, typically resolving within a few days Headache usually transient and manageable with standard analgesia Nausea or dizziness particularly in the initial treatment phase Skin reactions including itching, mild rash, or acneiform eruptions Temporary urine discolouration red or orange urine is harmless and expected Diarrhoea or flushing usually mild and self-limiting In patients with severe megaloblastic anaemia, low potassium levels (hypokalaemia) may occur during initial intensive treatment, but this is uncommon in routine maintenance therapy

Reversible dissociation of gamma-glutamylcysteine synthetase into two subunits