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Color reactions were quantified using a BioTek Synergy HT microplate reader at 490 nm

Common Symptoms of a Vitamin B12 Deficiency If you think you may be low, watch out for these symptoms: Feeling tired, fatigue and weakness Loss of appetite Constipation Weight loss Megaloblastic anemia Numbness and tingling Poor balance or coordination Confusion Memory loss Depression (21) Soreness of mouth and/or tongue (22) Be aware that many symptoms of B12 deficiency overlap with symptoms of other health conditions, so it's important to see your doctor for a definitive diagnosis

For each qPCR reaction, 25 l of master mix was prepared by adding 12.5 l of the 2 Universal SYBR (ThermoFisher) 5.5 l of RNAse-free water, 1 l ACX primer (0.05 g /ul), 1 l TS primer (0.1 g /l) (Table 1), and 4 l of the protein sample

SticozziCBelmonteGPecorelliAArezziniBGardiCMaioliEet alCigarette smoke affects keratinocytes Srb1 expression and localization via H2o2 production and hne protein adducts formation

For glutathione and glutamate quantification, cell viability analysis and oxidative stress assay, cells were sub-cultured in 12-well plates at a density of 4.2 10 5 cells/well