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The main metabolite, [ 3 H]proline (M1), accounted for 4.96% (female) and 3.93% (male) of the bile samples (Figure 5C)

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For the final stage of maturation into functional hepatocyte-like cells (HLCs), the proliferative HBs were cultured in hepatoZYME-SFM medium supplemented with 1X GlutaMAX