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Supplementation of butyrate (all Sigma-Aldrich, Germany) was done in the drinking water at a final concentration of 150 mM and changed every 3 days and control mice received pH and sodium-matched water
Animal-model evidence consistently shows local effects: angiogenesis at the injury site (VEGF upregulation), growth-factor signaling (EGF, FGF), nitric oxide pathway modulation, and accelerated tendon-to-bone interface healing in rat Achilles transection studies
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