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In vivo metabolic research employs diet-induced obesity mouse models (DIO C57BL/6) or genetic diabetic models (db/db, ob/ob) with controlled sema dosing and comprehensive metabolic phenotyping including indirect calorimetry, glucose and insulin tolerance tests, body composition by NMR or DXA, and tissue-level molecular profiling of liver, fat, and muscle samples
The average total weight loss at 68 weeks was 14.9%
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These models exhibit abnormal mitochondrial morphology in neurons, consistent with features of ferroptosis
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