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The previously described method for the induction of soft tissue injury was used here with some minor modifications [20]

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When administered directly into the cerebrospinal fluid of mice, it showed dose-dependent pain-relieving effects that were blocked by naloxone, confirming involvement of opioid pathways

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Increased NOX activity can lead to various pathologies, particularly cardiovascular diseases and neurodegenerative disorders (Bedard and Krause, 2007)