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Insulin was the first human protein manufactured using the method, approved for use in the treatment of diabetes in 1982

Toxicology research indicates the peptide has no lethal dose in rat models even at extremely high concentrations, and chronic administration studies show no organ toxicity or significant biochemical abnormalities

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By some measures, a dose of 1,500 mg/day was more effective than 3,000 mg/day, but by other measures the higher dose was more effective