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Cells were collected via dissociation by TrypLE Express and pelleted by centrifugation (300 x g , 3 min, room temperature in 15-ml conical tubes, followed by 21,000 x g , 2 min, room temperature in microcentrifuge tubes) and stored at -80C until further analysis

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The VEGFR2-independent mechanism involves Src kinase-mediated phosphorylation of caveolin-1, disrupting the inhibitory interaction between caveolin-1 and eNOS