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Briefly, cells were plated and 24 h later exposed to the indicated concentrations of FA

Other sirtuins, including SIRT2, SIRT4, SIRT5 and SIRT6, have a K m for NAD + below the physiological range, implying that NAD + might not necessarily be the rate-limiting of their activity

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Additionally, exercise work (J) and power (W) were calculated based on individual body weight using the equations: Rotarod performance test To evaluate motor coordination, balance, and skill learning, mice underwent a four-lane automated rotarod test (Fig