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Following the third wash, 10% of the bead volume was lysed with 1% Triton buffer for protein extracts and the remaining 90% was extracted in 80% methanol containing heavy labeled amino acid standards on a rotator shaker for 10 min at 4 C
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Many patients describe feeling controlled by food rather than making autonomous choices about eating
ERK1/2 phosphorylation assay FlpInCHO-hGLP-1R and FlpInCHO-pGLP-1R cells were seeded at a density of 3 10 4 cells/well into 96-well culture plates and incubated overnight at 37 C in 5% CO 2
Finally, CrAT might play a fundamental role in histone acetylation in cancer cells
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