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The HL60 cells were transfected with CHAC-targeting shRNA lentiviral particles (Sh-CHAC2) or control shRNA lentiviral particles (Sh-NC) using Lipofectamine 3000 (Invitrogen) according to the manufacturers instructions
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published in the Journal of Applied Physiology confirmed that BPC-157 significantly accelerated outgrowth of Achilles tendon explants, markedly increased in vitro tendon fibroblast migration in a dose-dependent manner, and substantially increased fibroblast survival under HO-induced oxidative stress establishing a three-part cellular mechanism (outgrowth, migration, survival) that distinguishes BPC-157s tendon repair profile from direct mitogenic agents
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