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Samples were resuspended in 160 L EPPS buffer (200 mM, pH 8.0) using a probe sonicator (1 1015 pulses, power output 20%) and then digested with LysC (4 L of 0.5 g/L per sample, resuspended in HPLC grade water, Wako-chemicals, Fujifilm #125-05061) for 2 h at 37 C in a shaker incubator
By reducing nicotinamide methylation, supports cellular NAD+ availabilitya critical coenzyme for mitochondrial function, energy metabolism, and cellular repair
Paracetamol or NSAIDs - Fast-acting pain relievers that ease headaches, muscle aches, and alcohol-induced inflammation without irritating the stomach
[1] Benzyl alcohol acts as a bacteriostatic agent, meaning it inhibits the growth of bacteria in the solution
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