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18 She had previously been managed with metformin, pioglitazone, and insulin, then was started on liraglutide (0.6mg/day titrated to 1.2mg/day) in combination with metformin
Tissues were then collected using a 100 m strainer, minced, and incubated at 37C for 30 minutes in a digestion solution containing DNase I (200 KU/mL
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