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3.3 scRNA-seq identifies FOXO signaling is the key mediator for tirzepatide induced -cell function improvement To elucidate the molecular mechanism underlying tirzepatide induced restoration of -cell function, we performed single cell RNA sequencing (scRNA seq) on islets isolated from chow fed (CTR), HFD fed (HFD), and 10nM tirzepatide treated (TZP) mice (Fig.4A)