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Using microscopy, we analysed the effects of 72 h 5FU treatment on mitochondrial morphology to identify potential organelle dysfunction
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The total weekly exposure remains identical to standard dosing
For metabolic analysis of cultured cancer cells and tumours, frozen tumour tissue was homogenized in 80% methanol containing 20 mM NEM using a Precellys cold tissue homogenizer (Bertin) at a ratio of 20 mg tissue to 1 ml solvent
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