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Animal studies involved naturally aged male C57BL/6 mice that were 20 months old and younger controls that were 3 and 13 months old
The cell pellet was resuspended and incubated for 30 min on ice with 25 L of staining mix in PBS containing 0.04% BSA, TruStain FcX Block (BioLegend 101320), and the mouse cell surface protein antibody panel containing the following oligo-conjugated anti-mouse antibodies (TotalSeq-A BioLegend) diluted 1:500: CD23 (Cat
Researchers often use a small volume of Acetic Acid 0.6% initially, followed by dilution with bacteriostatic water to achieve the desired final concentration
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