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Then pellets were resuspended in lysis buffer (Tris-HCl 100 mM and 0.5% Triton X-100, pH 8) containing Halt Protease and Phosphatase Inhibitor cocktail (Thermo Scientific, 1861280), thoroughly vortexed for 1 min and incubated for 15 min on ice
This classification allows the use of 5 amino 1mq capsules for in-vitro laboratory testing, experimental protocols and controlled scientific research
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Copper and ceruloplasmin levels assess copper status
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