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Cell viability and ROS and glutathione intracellular content of cells cultured in liquid medium were assessed by flow cytometry analysis of cells stained for 30 min in the dark with 0.025 M calcein-AM (ThermoFisher Scientific, C3099) at room temperature, 50 M Dichlorodihydrofluorescein-diacetate (DCFDA, ThermoFisher Scientific, D399) at 37 C, and 10 M monochlorobimane (ThermoFisher Scientific, M1381MP) at 37 C, respectively
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However, note that molecular sequence differences among GLP-1 analogs may impact efficacy and safety
The research tests a theory that states the human body's GLP-1 pathways function within joint tissues to control joint inflammation that occurs during osteoarthritis and rheumatoid arthritis
Do not take blood thinners for at least 2 weeks before the injections
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