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Description

NanoBiT assays were carried out as previously described (28): 24 hours after transfection, cells were incubated in 3 mM glucose for a minimum of 2 hours, detached and resuspended in NanoGlo Live cell substrate (N2012, Promega), 1:20 dilution in HBSS, and seeded onto an opaque white 96-well half-area plate

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Last Update: 18 May 2019

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DJW reports serving on data monitoring committees for Novo Nordisk not related to the topic of this work

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