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Cells were grown for 20 h before incubation in assay buffer (HBSS supplemented with 10 mM HEPES, 0.1% BSA or 0.1% casein, pH 7.4) for 30 min at 37 C
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Images were acquired in a charge-coupled device camera (Eagle), and gold particles quantified in at least five cells per experiment using Image J
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