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& Lotti, T

You might also reconsider thinking what is left? But the goal isnt a restriction forever

The isoelectric point calculator provides the pH at which the peptide net charge is zero

Site-directed mutagenesis Mutant forms of mito GCL refractory to the shRNA against GCL ( mito GCLmut) or catalytically inactive mito GCL ( mito GCL (E103A)) were generated using the site-directed mutagenesis QuikChange XL kit (Stratagene, La Jolla, CA, USA), followed by Dpn I digestion

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