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The pituitary ROI was centered at (MNI: x = 0 mm, y = 2 mm, z = 32 mm) [43] and expanded to cover nearby areas with high T1w image intensity (63 voxels, 0.50 mL)

It acts by stimulating the release of gonadotropin-releasing hormone (GnRH), which in turn triggers the secretion of luteinizing hormone (LH) and follicle-stimulating hormone (FSH)

Overexpression of miR-485-5p reverses this effect, confirming the functional specificity of this regulatory axis

Cell culture All cell lines (K7M2 (CRL-2836), 143B (CRL-8303), HOS (CRL-1543)) were obtained from the American Type Culture Collection (ATCC)