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The phosphate groups are usually added to serine, threonine, or tyrosine amino acids on the protein

The cocktail mixture/cleaving reagent in the present invention is selected from but not limited to HF, TFA (trifluoroacetic acid), TIS or TIPS (triisopropyl silane), Phenol, water, Anisole, Thioanisole, EDT (Ethane- 1,2-di thiol), 1 -dodecanethiol (DDT), Dithiothreitol (DTT), methanesulfonic acid or mixtures thereof

Cost and access remain significant barriers

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