Methylated & Non-methylated pUC19 DNA Set Name:Methylated & Non-methylated pUC19 DNA Set coli alkaline lysis method but

coli alkaline lysis method but using Zymolyase as the first solution

ZymoPURE P2 (Blue) is designed to be used with our ZymoPURE Plasmid Purification Kits

It features a highly specific Anti-5-Methylcytosine Monoclonal Antibody for the immunoprecipitation of methylated DNA in only a few hours

a unique ZymoTaq DNA Polymerase is included for robust amplification of bisulfite-treated DNA

Quick-DNA MagBinding Buffer for use with the Quick-DNA MagBead purification kits from Zymo Research

Methylated & Non-methylated pUC19 DNA Set Name:Methylated & Non-methylated pUC19 DNA Set coli alkaline lysis method butThe Methylated & Non methylated pUC19 DNA Set consists of control DNAs and a set of specifically designed primers. The set is ideal as a "spike in" control to assess bisulfite conversion efficiency within the same reaction as the sample, or to produce known mixtures of methylated and non methylated DNA for assay calibration. The non methylated pUC19 DNA is pUC19 isolated from a methylation negative strain of bacteria (Dam , Dcm ), and the methylated