Universal Methylated DNA Standard Name:Universal Methylated Human DNA Standard and Next-Generation sequencing

and Next-Generation sequencing

which cleaves phosphodiester bonds in DNA to yield homogeneous populations of core nucleosomes

bias also arises from sequencing library preparation and subsequent processes

This DNA standard is created by pooling DNA extracted from pure cultures

and rapid isolation of up to 1 mg total RNA from cultured cells or tissue samples

Universal Methylated DNA Standard Name:Universal Methylated Human DNA Standard and Next-Generation sequencingThe Universal Methylated DNA Standards are designed to be used as positive controls to assess the efficiency of DNA bisulfite conversion in human and mouse models. The control DNA can be run in parallel with samples to monitor the bisulfite conversion reaction. The primer sets are designed to amplify a fragment of the supplied DNA from the respective species following bisulfite treatment.