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The cells are now transformation ready
providing an unbiased way of calculating the efficiency of the bisulfite conversion reaction
end repair
PAC) from E
6 ml dry volume mixed 0
PureRec Duplex-Specific Nuclease (DSN) WGBS Library Kit The cells are now transformationPureRec Duplex Specific Nuclease (DSN) is an endonuclease that selectively digests double stranded DNA (dsDNA) and DNA in DNA RNA hybrids, exhibiting virtually no activity towards single stranded DNA (ssDNA) or RNA. It can discriminate between perfectly matched and non matched short DNA duplexes. To catalyze cleavage, DSN needs a minimum of 10 bp dsDNA or 15 bp perfectly matched DNA RNA duplex. This enzymatic function is reliant upon the presence of
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